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The myocilin (MYOC) gene expression in the human trabecular meshwork
H Takahashi1, S Noda, Y Mashima
1Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Current Eye Research
|January 5, 2000
Summary
The myocilin (MYOC) gene, also known as TIGR, is clearly expressed in trabecular meshwork cells. This finding holds true for both glaucomatous and non-glaucomatous eyes, suggesting its role in glaucoma pathogenesis.
Area of Science:
- Ophthalmology
- Genetics
- Cell Biology
Background:
- Myocilin (MYOC) is a novel cytoskeletal protein identified in photoreceptor connecting cilia.
- Myocilin is identical to TIGR (trabecular meshwork inducible glucocorticoid response protein), implicated in juvenile open-angle glaucoma pathogenesis.
Purpose of the Study:
- To investigate the expression of the myocilin (MYOC)/TIGR gene in the trabecular meshwork.
- To compare gene expression in glaucomatous versus non-glaucomatous eyes.
Main Methods:
- In situ RNA hybridization was performed on trabecular meshwork specimens.
- Specimens were obtained from patients with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG), steroid glaucoma, and control subjects.
Main Results:
- Hybridization signals for the myocilin gene were detected in trabecular meshwork cells.
- Secondary signals were observed in fibroblast-like cells of the corneoscleral wall.
Conclusions:
- The myocilin gene is demonstrably expressed in trabecular meshwork cells.
- Expression is consistent in both glaucomatous and non-glaucomatous eyes, indicating its constitutive presence.