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Acidic phospholipid bicelles: a versatile model membrane system.

J Struppe1, J A Whiles, R R Vold

  • 1Department of Chemistry, University of California San Diego, La Jolla, California 92093-0359 USA. ekomives@ucsd.edu

Biophysical Journal
|January 5, 2000
PubMed
Summary

Acidic bicelles, useful membrane models, were studied using deuterium NMR. While less stable, they show similar lipid ordering and peptide binding to neutral bicelles.

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Area of Science:

  • Biophysics
  • Membrane Biophysics
  • Lipid Bilayer Systems

Background:

  • Bicellar solutions are valuable model systems for studying biological membranes.
  • Understanding the properties of acidic bicelles is crucial for expanding their application range.
  • Dimyristoyl/dihexanoylphosphatidylcholine (DMPC/DHPC) bicelles are commonly used membrane mimetics.

Purpose of the Study:

  • To investigate the properties of acidic bicelles containing dimyristoylphosphatidylserine (DMPS) or dimyristoylphosphatidylglycerol (DMPG).
  • To assess the stability and structural characteristics of these novel membrane model systems.
  • To evaluate the utility of acidic bicelles in studying peptide-membrane interactions.

Main Methods:

  • Deuterium NMR spectroscopy was employed to analyze bicellar solutions.

Related Experiment Videos

  • Solid-state deuterium NMR was used to determine lipid ordering.
  • The binding of a specific peptide (mu-GSSKSKPKDPSQRR from pp60(nu-src)) was studied.

    • Main Results:

    • Acidic bicelles exhibit a reduced stability range (pH 5.5-7) compared to neutral bicelles (pH 4-7) due to lipid miscibility issues.
    • Deuterium NMR analysis revealed similar ordering of DMPC and the acidic lipids (DMPS/DMPG) in both neutral and acidic bicelles.
    • The studied peptide demonstrated binding to both neutral and acidic bicelles, indicating their potential as membrane mimetics.

    Conclusions:

    • Acidic bicelles, despite reduced stability, serve as effective membrane models.
    • Lipid ordering and peptide interactions are comparable between neutral and acidic bicelles.
    • These findings highlight the utility of acidic bicelles for biophysical studies.