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Dilute bicellar solutions for structural NMR work

J Struppe1, R R Vold

  • 1Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, California, 92093-0359, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|January 8, 1999
PubMed
Summary

Deuterium NMR reveals bicellar solutions align at lower concentrations than predicted. This alignment is influenced by lipid ratios and temperature, suggesting a dynamic equilibrium in bicelle formation.

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Area of Science:

  • Biophysics
  • Materials Science
  • Spectroscopy

Background:

  • Bicellar solutions are model membrane systems.
  • Onsager's model predicts phase stability based on mesogen size and concentration.
  • Previous models inadequately predict bicellar solution alignment.

Purpose of the Study:

  • Characterize concentration-dependent orientational order in DMPC/DHPC bicellar solutions.
  • Investigate the stability of the discotic nematic phase in these systems.
  • Determine the influence of lipid ratios (q) on bicellar order and phase behavior.

Main Methods:

  • Deuterium Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Viscosity measurements.
  • Analysis of bicellar morphology across varying concentrations, temperatures, and q-values.

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Main Results:

  • Macroscopic alignment observed at concentrations significantly lower than Onsager's model predictions.
  • Discotic nematic phase stable at 3-5% (w/w) for q = 3.3-2.3.
  • Bicellar order peaks just before phase separation; viscosity indicates morphological changes below 30°C.

Conclusions:

  • Onsager's model is insufficient for predicting bicellar solution alignment.
  • A DHPC bicelle/solution equilibrium likely extends alignment concentration range.
  • Temperature significantly impacts bicellar morphology and solution properties.