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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus
1Oxford Centre for Molecular Sciences, Dyson Perrins Laboratory, South Parks Road, Oxford, OX1 3QY, United Kingdom.
Biochemical and Biophysical Research Communications
|January 7, 2000
Abstract:
Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthase were mutated to alanine residues. The C100A mutant had properties similar to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 reduced enzyme activity with penicillin N and penicillin G to different extents.
