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Boldine action against the stannous chloride effect
I W Reiniger1, C Ribeiro da Silva, I Felzenszwalb
1Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofísica e Biometria, Brazil.
Journal of Ethnopharmacology
|January 7, 2000
Summary
Boldine, derived from Peumus boldus, protects Escherichia coli from oxidative stress caused by stannous chloride. This antioxidant effect may involve boldine reacting with stannous ions, preventing reactive oxygen species generation.
Area of Science:
- Pharmacology and Toxicology
- Molecular Biology
- Antioxidant Research
Background:
- Peumus boldus extract, containing boldine, is traditionally used for liver and biliary ailments.
- Oxidative stress, induced by reactive oxygen species (ROS), can damage cellular components.
- Stannous chloride is known to induce ROS generation via Fenton-like reactions.
Purpose of the Study:
- To investigate the protective effects of boldine against stannous chloride-induced oxidative stress in Escherichia coli.
- To assess boldine's influence on plasmid structural conformation under oxidative conditions.
Main Methods:
- Assessed the survival rate of Escherichia coli AB1157 exposed to stannous chloride-induced ROS in the presence and absence of boldine.
- Analyzed the structural integrity of plasmid pUC 9.1 using gel electrophoresis following exposure to stannous chloride and boldine.
Main Results:
- Boldine significantly reduced the lethal effects of stannous chloride on E. coli survival.
- Boldine did not alter the supercoiled structure of the plasmid pUC 9.1 when exposed to stannous chloride.
- Results suggest an antioxidant mechanism for boldine's protective action.
Conclusions:
- Boldine exhibits antioxidant properties, protecting E. coli from stannous chloride-induced oxidative damage.
- The protective effect is likely due to boldine's interaction with stannous ions, inhibiting ROS formation.
- Boldine demonstrates potential as a protective agent against oxidative stress.
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