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Chromosomal beta-lactamase is packaged into membrane vesicles and secreted from Pseudomonas aeruginosa
O Ciofu1, T J Beveridge, J Kadurugamuwa
1Institute for Medical Microbiology and Immunology, Panum Inst. 24.1, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark. O.Ciofu@immi.ku.dk
The Journal of Antimicrobial Chemotherapy
|January 11, 2000
Abstract:
Membrane vesicles were isolated from one beta-lactam-sensitive and three beta-lactam-resistant Pseudomonas aeruginosa clinical isolates from patients with cystic fibrosis. The presence of the chromosomally encoded beta-lactamase in the membrane vesicles was shown by electron microscopy and enzymatic studies. This is the first report of extracellular secretion of beta-lactamase in P. aeruginosa and it seems that the enzyme is packaged into membrane vesicles.