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Microbial iron chelates with iron donor properties in hemoglobin-synthesizing cells
Experimental Hematology
|March 1, 1976
Abstract:
Iron incorporation into Friend virus infected leukemic murine spleen cells was studied using the two fungal iron trihydroxamates, fusigen and ferricrocin. Incorporation of 55Fe was measured by isolation of hemoglobin after dimethylsulfoxide-induced hemoglobin synthesis and compared with iron incorporation from 55Fe-labeled ferric citrate.
Insights
This study investigated iron uptake in Friend virus-infected mouse spleen cells. Fungal iron compounds, fusigen and ferricrocin, showed comparable iron incorporation to ferric citrate for hemoglobin synthesis.
Area of Science:
- Biochemistry
- Hematology
- Virology
Background:
- Friend virus infection induces leukemia in mice, affecting spleen cell function.
- Iron metabolism is crucial for hemoglobin synthesis, a key indicator of erythropoiesis.
Purpose of the Study:
- To compare the efficacy of fungal iron trihydroxamates (fusigen, ferricrocin) versus ferric citrate in iron incorporation into leukemic murine spleen cells.
- To assess the role of these iron compounds in supporting dimethylsulfoxide-induced hemoglobin synthesis.
Main Methods:
- Utilized radioactive 55Fe labeling to trace iron incorporation.
- Isolated hemoglobin from Friend virus-infected murine spleen cells.
- Induced hemoglobin synthesis using dimethylsulfoxide (DMSO).
Main Results:
- Both fusigen and ferricrocin facilitated 55Fe incorporation into spleen cells.
- Iron incorporation from fungal trihydroxamates was comparable to that from ferric citrate.
- Successful hemoglobin isolation confirmed iron utilization for synthesis.
Conclusions:
- Fungal iron trihydroxamates are effective in delivering iron for hemoglobin synthesis in leukemic murine spleen cells.
- These compounds represent potential alternatives for iron supplementation in related cellular models.