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Injectable dextranomer-based implant: histopathology, volume changes and DNA-analysis.
A Stenberg1, E Larsson, A Lindholm
1Department of Women's and Children's Health, University Hospital, Uppsala, Sweden.
Scandinavian Journal of Urology and Nephrology
|January 15, 2000
Summary
Dextranomers in sodium hyaluronan solution (DiHA) showed minimal tissue reaction and no DNA changes in animal studies, indicating its safety and suitability as a tissue-augmenting injectable substance.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Dextranomers in sodium hyaluronan solution (DiHA) is a novel injectable substance.
- Understanding its tissue interaction is crucial for clinical application.
Purpose of the Study:
- To evaluate the tissue reaction and implant volume changes of DiHA.
- To assess the DNA profile of cells invading DiHA implants.
Main Methods:
- DiHA was injected into pigs (bladder) and rats (subcutaneous tissue).
- Histopathological analysis, DNA profiling, and implant volume measurements were performed over extended follow-up periods.
Main Results:
- Early cellular infiltration (fibroblasts, inflammatory cells, giant cells) was observed.
- Later stages showed increased extracellular matrix and vascularization without necrosis.
- No aneuploid cells were detected; implant volume decreased by 23% over 12 months.
Conclusions:
- DiHA demonstrates minimal adverse tissue reactions.
- No genotoxicity was observed, supporting its safety profile.
- DiHA is a safe and effective injectable material for tissue augmentation.