Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Targeting DNA with triplexes.

K R Fox1

  • 1Division of Biochemistry & Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, UK. krf1@soton.ac.uk

Current Medicinal Chemistry
|January 19, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Randomized evaluation of an online single-session intervention for minority stress in LGBTQ+ adolescents.

Internet interventions·2023
Same author

Psychosis as a risk factor for suicidal thoughts and behaviors: a meta-analysis of longitudinal studies.

Psychological medicine·2017
Same author

The Blood Donation Ambivalence Survey: measuring conflicting attitudes about giving blood.

Transfusion medicine (Oxford, England)·2017
Same author

Perceptions of success of a local UK public health collaborative†.

Health promotion international·2017
Same author

Biological risk factors for suicidal behaviors: a meta-analysis.

Translational psychiatry·2016
Same author

Letter to the Editor: Suicide as a complex classification problem: machine learning and related techniques can advance suicide prediction - a reply to Roaldset (2016).

Psychological medicine·2016
Same journal

Screening of Medicinal and Edible Homology Substances for Diabetic Kidney Disease Based on GraphBAN.

Current medicinal chemistry·2026
Same journal

Berberine as an Antimicrobial Agent and Gut Microbiota Modulator: Mechanisms and Therapeutic Potential.

Current medicinal chemistry·2026
Same journal

Research Progress of Grapefruit-derived Extracellular Vesicles in Anti-tumor Treatment.

Current medicinal chemistry·2026
Same journal

Corrigendum to: Neuroprotective Mechanisms of Oxygen and Ethanol: A Potential Combination Therapy in Stroke.

Current medicinal chemistry·2026
Same journal

Ferroptosis and Its Crosstalk with Other Cell Death Modes in Ischemic Stroke.

Current medicinal chemistry·2026
Same journal

Integrative Analysis Reveals BPTF, COL1A1, and COL4A2 as Fibroblast-Related Biomarkers Associated with Immune Infiltration in Ovarian Cancer.

Current medicinal chemistry·2026
See all related articles

Researchers explored DNA triple helix formation for antigene agents. Strategies were developed to overcome pH-dependent binding and improve stability for enhanced molecular biology tools.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Intermolecular DNA triple helices offer extensive sequence recognition for antigene agents and molecular biology tools.
  • A third oligonucleotide strand binds in the DNA major groove, forming specific contacts with base pairs.
  • Existing triplex structures face limitations including low pH requirements for C+*GC triplets and lower stability compared to duplex DNA.

Purpose of the Study:

  • To review strategies for overcoming limitations in DNA triple helix formation.
  • To address the pH dependency of C+*GC triplet formation.
  • To enhance the stability of DNA triple helix structures.

Main Methods:

  • Investigating DNA base analogues for pH-independent GC recognition.
  • Evaluating novel base analogues to improve triplex stability.

Related Experiment Videos

  • Exploring backbone modifications for enhanced triplex stability.
  • Examining the use of triplex-specific binding ligands to increase stability.

    • Main Results:

    • Various DNA base analogues have been employed to achieve pH-independent recognition of GC base pairs.
    • Novel base analogues, backbone modifications, and specific binding ligands can increase triplex stability.
    • Each analogue presents unique benefits and limitations for triplex formation.

    Conclusions:

    • Strategies involving base analogues, backbone modifications, and ligands can overcome pH and stability limitations of DNA triple helices.
    • These advancements expand the potential of DNA triple helices as antigene agents and molecular biology tools.
    • Further research into these modifications can optimize triplex design for specific applications.