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Related Experiment Videos

Crystal polymer interaction with new injectable bone substitute; SEM and Hr TEM study.

G Daculsi1, R Rohanizadeh, P Weiss

  • 1UPRES EA 2159, Faculté de Chirurgie Dentaire, Place A Ricordeau, 44 042 Nantes, France.

Journal of Biomedical Materials Research
|January 25, 2000
PubMed
Summary

Injectable bone substitute (IBS) interactions between calcium phosphate (CaP) and hydroxypropyl methylcellulose (HPMC) were studied. Strong chemical interactions were observed at the nanoscale, confirming IBS

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Materials Chemistry

Background:

  • Injectable bone substitutes (IBS) are advanced materials for bone regeneration.
  • Calcium phosphate (CaP) and hydrophilic polymers like hydroxypropyl methylcellulose (HPMC) are key components.
  • Understanding organic-inorganic interactions is crucial for optimizing IBS performance.

Purpose of the Study:

  • To investigate the chemical interactions between CaP granules and HPMC in an injectable bone substitute (IBS).
  • To characterize the nanoscale changes occurring at the interface of CaP and HPMC.
  • To evaluate the stability of these interactions under different conditions.

Main Methods:

  • Development of a ready-to-use, sterile CaP/HPMC composite (IBS).

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  • Analysis using Scanning Electron Microscopy (SEM) for morphology.
  • Elemental analysis with Energy Dispersive X-ray spectroscopy (EDX).
  • High-Resolution Transmission Electron Microscopy (HrTEM) for nanoscale structural analysis.

    • Main Results:

    • SEM showed CaP granule degradation into smaller particles.
    • EDX indicated no significant change in the Ca/P ratio over time.
    • HrTEM revealed nanoscale hydrolysis (dissolution/precipitation) within hydroxyapatite (HA) crystals up to 13 nm.
    • Enlarged lattice distances in HA suggest HPO(4) diffusion.
    • Beta-tricalcium phosphate (beta-TCP) crystals showed surface dissolution.

    Conclusions:

    • Strong nanoscale chemical interactions occur between HPMC and CaP in IBS.
    • These interactions are confined to the outermost nanometers of the CaP crystals.
    • The observed hydrolysis and diffusion mechanisms are key to the composite's behavior.
    • The composite demonstrated stability over time and after steam sterilization.