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Adenosine modulates the (Na(+)+K(+))ATPase activity in malpighian tubules isolated from Rhodnius prolixus

C Caruso-Neves1, S O Monteiro, C F de Oliveira

  • 1Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Archives of Insect Biochemistry and Physiology
|January 25, 2000
PubMed

Insights

Adenosine inhibits sodium-potassium pump activity in Rhodnius prolixus Malpighian tubules. This effect is mediated by the A(1) adenosine receptor, highlighting its role in regulating ion transport.

Area of Science:

  • Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • The (Na(+)+K(+))ATPase is crucial for ion transport in insect Malpighian tubules.
  • Adenosine signaling pathways are involved in various physiological processes in insects.

Purpose of the Study:

  • To investigate the regulatory role of adenosine on (Na(+)+K(+))ATPase activity in Rhodnius prolixus Malpighian tubules.
  • To determine the specific adenosine receptor subtype involved in this regulation.

Main Methods:

  • Isolated Malpighian tubules from Rhodnius prolixus were used.
  • Enzyme activity assays were performed to measure (Na(+)+K(+))ATPase specific activity.
  • Specific adenosine receptor agonists and antagonists were employed to probe receptor involvement.

Main Results:

  • Adenosine significantly decreased (Na(+)+K(+))ATPase specific activity in a dose-dependent manner (maximal inhibition of 88% at 10(-9) M).
  • N(6)-cyclohexyladenosine (CHA), an A(1) receptor agonist, mimicked adenosine's effect, while 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), an A(1) antagonist, reversed it.
  • Agonists and antagonists for the A(2) adenosine receptor did not affect enzyme activity or adenosine's inhibitory effect.

Conclusions:

  • The findings strongly suggest that adenosine inhibits (Na(+)+K(+))ATPase activity in Rhodnius prolixus Malpighian tubules.
  • This inhibition is specifically mediated through the activation of the A(1) adenosine receptor.

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