Transdentinal cell photobiomodulation using different wavelengths

Area of Science:

• Biomedical Engineering
• Cell Biology
• Dental Materials Science

Background:

• Odontoblast-like cells are crucial for dentin repair and regeneration.
• Transdentinal irradiation is a potential therapeutic approach in dentistry.
• Optimizing light-emitting diode (LED) parameters is key for effective photobiomodulation.

Purpose of the Study:

• To investigate the impact of transdentinal LED irradiation with varying parameters on odontoblast-like cells (MDPC-23).
• To determine the optimal LED wavelengths and energy densities for stimulating cellular responses.

Main Methods:

• Human dentin discs were irradiated with LEDs (450, 630, 840 nm) at different energy densities (0, 4, 25 J/cm²).
• Evaluated cell viability, alkaline phosphatase (ALP) activity, total protein synthesis (TP), and cell morphology.
• Analyzed collagen type I (Col-I) gene expression using quantitative PCR.

Main Results:

• LED irradiation at 630 nm and 25 J/cm² significantly increased cell viability (21.8%).
• Increased ALP activity was observed across most tested LED parameters, except 450 nm at 4 J/cm².
• Infrared LED at 4 J/cm² enhanced Col-I gene expression, while higher energy densities promoted cell attachment.

Conclusions:

• Infrared LED irradiation (4 J/cm²) and red LED irradiation (25 J/cm²) are effective for transdentinal photobiomodulation.
• These parameters show promise for stimulating odontoblast-like cell functions in dental regenerative therapies.