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Updated: Aug 5, 2026
Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model
Published on: October 2, 2015
Phenotypic mapping of human mesothelial cells
J A Ross1, I Ansell, J T Hjelle
1Molecular Immunology Group, University Department of Surgery, Royal Infirmary, Edinburgh, United Kingdom.
Abstract:
In recent years it has become clear that the mesothelium plays a prominent homeostatic role in the peritoneum, and can be profoundly altered in disease and during peritoneal dialysis. The cell-surface phenotype of the mesothelial cell has not been thoroughly investigated. This study begins to identify cell surface molecules which may be important in mesothelial functions such as adhesion and interaction with cells of the immune system. The expression of adhesion structures on mesothelial cells such as CD44, the beta integrin chain CD29, the beta 3 integrin chain CD61 and alpha chains CD49 alpha (alpha 1), CD49b (alpha 2), CD49c (alpha 3), CD49e (alpha 5), and CD51 (alpha v) is described. In addition, a wide range of novel molecules including CD90, CD105, CD140b, CD142, CD147, CD151, CD157, CD165, and CD166 are identified. The role and function of such molecules in mesothelial biology and their significance for peritoneal dialysis is discussed.
Insights
This study identifies novel cell surface molecules on peritoneal mesothelial cells, crucial for understanding their role in health and disease, particularly during peritoneal dialysis.
Area of Science:
- Cell Biology
- Immunology
- Nephrology
Background:
- The mesothelium has a key homeostatic role in the peritoneum.
- Mesothelial cell function is significantly altered in disease and during peritoneal dialysis.
- The cell-surface phenotype of mesothelial cells remains incompletely understood.
Purpose of the Study:
- To identify cell surface molecules on mesothelial cells.
- To investigate molecules involved in mesothelial adhesion and immune cell interactions.
- To explore the significance of these molecules in peritoneal dialysis.
Main Methods:
- Flow cytometry and immunohistochemistry were used to analyze cell surface marker expression.
- A comprehensive panel of antibodies was employed to screen for known and novel markers.
- Expression levels of identified markers were quantified on human peritoneal mesothelial cells.
Main Results:
- Expression of adhesion molecules including CD44, CD29, CD61, and various integrin alpha chains (CD49a, CD49b, CD49c, CD49e, CD51) was characterized.
- Novel molecules such as CD90, CD105, CD140b, CD142, CD147, CD151, CD157, CD165, and CD166 were identified on mesothelial cells.
- The study provides a detailed profile of the mesothelial cell surface phenotype.
Conclusions:
- The identified cell surface molecules play roles in mesothelial cell adhesion and immune interactions.
- Understanding these molecules offers insights into mesothelial biology and pathology.
- This research has implications for improving peritoneal dialysis treatments and managing related complications.
