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Related Experiment Videos

Volume dynamics in migrating epithelial cells measured with atomic force microscopy.

S W Schneider1, P Pagel, C Rotsch

  • 1Physiologisches Institut, Münster, Germany.

Pflugers Archiv : European Journal of Physiology
|January 29, 2000
PubMed
Summary

Atomic force microscopy (AFM) precisely measures cell volume changes in migrating kidney cells. This technique reveals how IK channel activity causes localized cell shrinkage, supporting cell migration mechanisms.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Renal Cell Physiology

Background:

  • Cell migration is crucial for tissue development and repair.
  • Transformed renal epithelial cells (MDCK-F cells) utilize a Ca(2+)-sensitive K+ channel (IK channel) for migration.
  • Previous studies hypothesized IK channel activity induces rear-end cell shrinkage, but direct volume measurement was challenging.

Purpose of the Study:

  • To develop and apply atomic force microscopy (AFM) for measuring MDCK-F cell volume in their native environment.
  • To demonstrate the dependence of cell volume on IK channel activity.
  • To visualize localized volume changes associated with IK channel function.

Main Methods:

  • Utilized atomic force microscopy (AFM) to create 3D images of MDCK-F cells.

Related Experiment Videos

  • Calculated cell volume by summing pixel-defined columns from AFM topographical data.
  • Measured volume changes upon IK channel blockade (charybdotoxin) and activation (ionomycin).

    • Main Results:

    • AFM successfully measured the mean volume of MDCK-F cells (2500+/-300 fl).
    • IK channel blockade with charybdotoxin increased cell volume by 17+/-4%.
    • IK channel activation via ionomycin decreased cell volume by 19+/-3%.
    • Volume changes (swelling and shrinkage) were predominantly observed at the rear of the cells.

    Conclusions:

    • AFM is a viable technique for measuring living cell volumes in physiological conditions.
    • IK channel activity directly influences MDCK-F cell volume, causing localized shrinkage at the cell rear.
    • These findings support the hypothesis that polarized IK channel activity drives cell migration through cytoskeletal mechanisms.