Search research articles

Search research articles

Related Experiment Videos

Cryo-planing for cryo-scanning electron microscopy.

J Nijsse¹, A C van Aelst

¹Department of Plant Sciences, Wageningen University, The Netherlands.

|February 2, 2000

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High viscosity and anisotropy characterize the cytoplasm of fungal dormant stress-resistant spores.

Eukaryotic cell·2006

Same author

Visualization studies of human skin in vitro/in vivo under the influence of an electrical field.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2006

Same author

Field-emission scanning electron microscopy analysis of morphology and enzyme distribution within an industrial biocatalytic particle.

Scanning·2005

Same author

Combined chemical and electrical enhancement modulates stratum corneum structure.

Journal of controlled release : official journal of the Controlled Release Society·2003

Same author

An antifungal compound produced by Bacillus subtilis YM 10-20 inhibits germination of Penicillium roqueforti conidiospores.

Journal of applied microbiology·2003

Same author

Viability loss of neem (Azadirachta indica) seeds associated with membrane phase behaviour.

Journal of experimental botany·2001

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Cryo-planing techniques for low-temperature scanning electron microscopy (cryo-SEM) yield flat sample surfaces. This study reviews, compares, and enhances these methods for improved cryo-SEM, X-ray microanalysis, and image analysis.

Area of Science:

Materials Science
Microscopy Techniques
Biological Sample Preparation

Background:

Advancements in cryo-planing have enabled flat sample surface preparation for low-temperature scanning electron microscopy (cryo-SEM).
Stable specimen attachment is crucial for successful cryo-planing, often achieved with screw mechanisms and indium as a space-filler.

Purpose of the Study:

To review and compare existing cryo-planing techniques for cryo-SEM sample preparation.
To introduce modifications for improved cryo-planing results.
To demonstrate the application of cryo-planing on diverse biological materials.

Main Methods:

Review of established cryo-planing methods: cryo-milling, cold-room microtomy, and cryo-ultramicrotomy.
Development and implementation of modified cryo-planing techniques.

Related Experiment Videos

Application of techniques to chrysanthemum, hazel, and rapeseed samples.

Main Results:

Successful cryo-planing achieved using cryo-milling, microtomy, or cryo-ultramicrotomy after addressing specimen attachment.
Modified cryo-planing produces flat surfaces on frozen, fully hydrated samples at any desired plane.
Demonstrated effectiveness on fibrous, hard, and delicate plant tissues.

Conclusions:

Cryo-planing modifications enhance the preparation of flat surfaces for frozen, hydrated biological samples.
Improved sample surfaces benefit cryo-SEM, X-ray microanalysis, and digital image analysis.
The presented techniques are versatile, applicable to a range of material types.