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Related Experiment Videos

Sry induces cell proliferation in the mouse gonad.

J Schmahl1, E M Eicher, L L Washburn

  • 1The Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.

Development (Cambridge, England)
|February 2, 2000
PubMed
Summary

The Sry gene triggers rapid somatic cell proliferation in developing XY gonads, initiating testis formation. This early increase in cell division is the first identified consequence of Sry gene expression.

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Evidence that Sry is expressed in pre-Sertoli cells and Sertoli and granulosa cells have a common precursor.

Developmental biology·2002

Area of Science:

  • Developmental Biology
  • Genetics
  • Reproductive Biology

Background:

  • The Sry gene on the Y chromosome is essential for mammalian testis development.
  • Early morphological changes in XY gonads include a size increase compared to XX gonads.

Purpose of the Study:

  • To identify the earliest cellular events following Sry gene expression.
  • To investigate the role of Sry in gonad size increase and cell proliferation.

Main Methods:

  • Utilized 5'-bromo-2'-deoxyuridine (BrdU) incorporation to label dividing cells.
  • Compared cell proliferation rates in XY and XX gonads.
  • Analyzed proliferation in SF1-positive and SF1-negative cells.

Main Results:

  • XY gonads showed a dramatic, male-specific increase in somatic cell proliferation.
  • Proliferation occurred in two stages: SF1-positive cells (contributing to Sertoli cells) and SF1-negative cells.
  • This proliferation began within 24 hours of Sry expression, preceding other male-specific changes.

Conclusions:

  • Increased cell proliferation in the male coelomic epithelium is the earliest identified effect of Sry.
  • Sry-dependent proliferation is crucial for initiating testis formation.
  • This process is independent of other genetic differences between XY and XX gonads.

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