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Developmental change in TATA-box utilization during preimplantation mouse development.

W Davis1, R M Schultz

  • 1Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018, USA.

Developmental Biology
|February 5, 2000
PubMed
Summary
This summary is machine-generated.

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During mouse preimplantation development, gene expression is reprogrammed. The study reveals a shift in promoter usage for the eIF-1A gene from TATA-containing to TATA-less promoters, crucial for embryonic genome activation.

Area of Science:

  • Developmental Biology
  • Gene Regulation
  • Molecular Biology

Background:

  • Embryonic genome activation (EGA) involves significant gene expression reprogramming.
  • The eukaryotic translation initiation factor 1A (eIF-1A) gene is regulated by two promoters: a proximal TATA-containing and a distal TATA-less promoter.

Purpose of the Study:

  • To investigate the dynamic changes in promoter utilization for the eIF-1A gene during mouse preimplantation development.
  • To determine the role of embryonic transcription in this promoter shift.

Main Methods:

  • Specific amplification of cDNA ends (SACE) to differentiate transcripts from TATA-less and TATA-containing promoters.
  • Culture of one-cell embryos with alpha-amanitin to inhibit embryonic transcription.

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Main Results:

  • In oocytes, 70% of eIF-1A transcripts originate from the TATA-containing promoter.
  • By the two-cell stage, transcript origin shifts to 75% from the TATA-less promoter.
  • Inhibition of embryonic transcription prevents this promoter shift, maintaining oocyte-like distribution.

Conclusions:

  • Promoter utilization for the eIF-1A gene dramatically shifts from TATA-containing to TATA-less promoters during EGA.
  • This shift is dependent on embryonic transcription and may reflect a broader change in promoter usage during the maternal-to-zygotic transition.
  • Altered promoter usage contributes to the global reprogramming of gene expression essential for early development.