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Related Experiment Videos

The effect of supramaximal exercise on equine platelet function.

J K Kingston1, S N Sampson, L A Beard

  • 1Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164-6610, USA.

Equine Veterinary Journal. Supplement
|February 5, 2000
PubMed
Summary

Higher sodium citrate concentrations in equine blood samples inhibit platelet aggregation. Low molecular weight heparin offers a reliable alternative for assessing platelet function during strenuous exercise, revealing enhanced platelet aggregability post-exercise.

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Area of Science:

  • Veterinary Medicine
  • Hematology
  • Exercise Physiology

Background:

  • Sodium citrate anticoagulant concentration varies with packed cell volume (PCV), affecting platelet aggregation.
  • Horses exhibit elevated PCV during high-intensity exercise, potentially altering citrate anticoagulant efficacy.
  • Accurate assessment of equine platelet function during exercise is crucial for understanding physiological responses.

Purpose of the Study:

  • To investigate the impact of varying plasma sodium citrate concentrations on equine platelet aggregability.
  • To evaluate low molecular weight heparin (LMWH) as an alternative anticoagulant for equine platelet function tests.
  • To determine the effect of supramaximal exercise on equine platelet aggregability.

Main Methods:

  • Blood samples collected pre- and post-exercise from horses undergoing treadmill exercise.

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  • Samples anticoagulated with either sodium citrate (9:1 ratio) or LMWH.
  • Platelet aggregation induced by adenosine diphosphate (ADP) measured using optical aggregometry.

    • Main Results:

    • Sodium citrate samples showed significantly reduced platelet aggregability post-exercise compared to pre-exercise.
    • Adjusting pre-exercise sodium citrate concentrations to match post-exercise levels mimicked the inhibitory effect.
    • LMWH-anticoagulated samples demonstrated significantly increased platelet aggregability post-exercise.

    Conclusions:

    • Elevated plasma sodium citrate concentrations significantly inhibit equine platelet aggregation.
    • LMWH is a suitable anticoagulant for evaluating equine platelet function during strenuous exercise.
    • Equine platelet aggregability is enhanced following supramaximal exercise.