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Published on: July 4, 2015
Mechanoluminescence: lymphocyte analysis after exposure to ionizing radiation
V E Orel1, I N Kadyuk, N N Dzyatkovskaya
1Physics-Technical Laboratory, Ukrainian Research Institute of Oncology and Radiology, Lomonosova St. 33/43, 252022 Kyiv-22, Ukraine. orel@ucr.kiev.ua
Abstract:
Lymphocyte mechanoluminescence (ML) develops as a result of mechanochemical activation of cells. This paper describes devices (tribogenerators) and techniques for lymphocyte mechanoluminescence analysis. At different stages of the cellular cycle the ML of non-irradiated peripheral blood lymphocytes (PBL) showed characteristic differences. After (60)Co gamma -irradiation of PBL in vitro at 0.25 Gy and 1 Gy an alteration of ML was observed with a relationship between ML values of lymphocytes, and also of mitochondria (MT), and the dose of ionizing radiation used. By using bioluminescence techniques it was shown that gamma-irradiation reduces the amount of ATP synthesized by PBL in the stationary phase.
