STUDY OF THE COMBINED EFFECT OF DOXORUBICIN-LOADED SUPERPARAMAGNETIC NANOPARTICLES AND NON-IONISING ELECTROMAGNETIC...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Oncology

Background:

• Superparamagnetic nanoparticles offer targeted drug delivery.
• Electromagnetic irradiation can enhance therapeutic effects.
• Walker256 carcinosarcoma is a model for experimental cancer research.

Purpose of the Study:

• To investigate the combined efficacy of doxorubicin-loaded superparamagnetic nanoparticles and electromagnetic irradiation against Walker256 carcinosarcoma.
• To evaluate the impact of this magnetochemical technology on tumor growth, heterogeneity, and redox state.

Main Methods:

• Walker256 carcinosarcoma was induced in rats.
• Animals received doxorubicin-loaded superparamagnetic Fe3O4 nanoparticles and were exposed to static magnetic and electromagnetic fields.
• Tumor structural changes were assessed using MRI and texture analysis.
• Redox markers (free iron, ceruloplasmin, superoxide radical, nitric oxide) were measured using electron paramagnetic resonance spectroscopy.

Main Results:

• Combined treatment reduced tumor volume by 44-64% compared to controls.
• Texture analysis revealed decreased intratumoral heterogeneity.
• Electron paramagnetic resonance spectroscopy showed altered levels of superoxide radical, nitric oxide, and free iron in tumors and blood.

Conclusions:

• Doxorubicin-loaded superparamagnetic nanoparticles combined with electromagnetic irradiation effectively inhibit Walker256 carcinosarcoma growth.
• This approach reduces intratumoral heterogeneity.
• The treatment induces significant changes in the tumor and blood redox state.