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Macro approach and fuzzy modeling of entrapped biocatalyst
1Dipartimento di Scienza dei Materiali e Ingegneria Chimica, Politecnico di Torino, C.(so) Duca degli Abruzzi 24, 10129 Torino, Italy. ruggeri@polito.it
Abstract:
The interactions between a strain of Saccharomyces cerevisiae and an alginate matrix are investigated to ascertain the main factors affecting the bioreaction evolution. During the tests several parameters (glucose, ethanol, calcium ion and biomass concentration, pH, and alginate bed diameter) were evaluated, coupled with microscopic investigation inside the beads to determine the spatial biomass distribution. A detailed analysis of macro parameters and a correlation among them are proposed using a fuzzy algorithm. A global two-step fuzzy model results in which biomass distribution inside the beads is represented as a hidden parameter.