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Updated: Aug 31, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Autoinducer-2-associated synergy enhances fermentation, stability, and bioactive metabolite production in probiotic
Mei Bai1, Shuxu Guo1, Kaiming Wang1
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, PR China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, PR China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering; Inner Mongolia Agricultural University, Hohhot 010018, PR China; Collaborative Innovative Center of Ministry of Education for Lactic Acid Bacteria and Fermented Dairy Products, Inner Mongolia Agricultural University, Hohhot 010018, PR China.
Abstract:
This study investigates how autoinducer-2-mediated quorum sensing influences fermented milk performance. A high-autoinducer-2 base starter (Lactobacillus delbrueckii subsp. bulgaricus XJ51-2, Streptococcus thermophilus MGC23-8) combined with probiotics (Bifidobacterium animalis subsp. lactis BX246, Lactobacillus kefiranofaciens MGE42-8) formed seven starter variants. The four-strain blend demonstrated the shortest fermentation time (7.1 ± 0.1 h), highest viscosity (2375 mPa·s), earliest gelation (4.4 h), and viability >8.7 log CFU/mL after 21 days at 4 °C. Micro-rheology confirmed accelerated gel network formation. Untargeted metabolomics revealed enrichment of bioactive metabolites including glycerophospholipids, exopolysaccharide precursors, phenyllactate, hydroxycinnamates, organic acids, and short-chain fatty acids. These compounds enhance texture and flavor while offering antimicrobial and potential gut health benefits. Our findings suggest that autoinducer-2-associated interactions improve the technological and functional properties of fermented milk, providing a strategy for designing next-generation probiotic dairy products pending functional validation.
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