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Related Experiment Videos

Ethylammonium nitrate: a protein crystallization reagent.

J A Garlitz1, C A Summers, R A Flowers

  • 1Department of Chemistry, The University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606-3390, USA.

Acta Crystallographica. Section D, Biological Crystallography
|February 10, 2000
PubMed
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Ethylammonium nitrate (EAN) is a versatile liquid organic salt with applications in protein chemistry and crystallography. This study discusses its use as a precipitating agent for lysozyme crystallization.

Area of Science:

  • Protein chemistry and crystallography

Background:

  • Ethylammonium nitrate (EAN) is a liquid organic salt with unique hydrophobic, ionic, and hydrogen bonding properties.
  • These characteristics make EAN suitable for various applications in protein science, including use as an additive, detergent, or precipitating agent.

Purpose of the Study:

  • To explore the utility of ethylammonium nitrate (EAN) in protein crystallography.
  • To demonstrate the application of EAN as a precipitating agent for protein crystallization, specifically using lysozyme.

Main Methods:

  • Utilizing ethylammonium nitrate (EAN) as a precipitating agent.
  • Investigating the crystallization of lysozyme in the presence of EAN.

Main Results:

  • Ethylammonium nitrate (EAN) facilitates the crystallization of proteins.

Related Experiment Videos

  • Successful crystallization of lysozyme was achieved using EAN.

    • Conclusions:

    • Ethylammonium nitrate (EAN) is a promising solvent for protein crystallography.
    • EAN's properties enable its use in various protein structure determination techniques, including ligand delivery and precipitation-based crystallization.