Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence specificity of hydrogen-bonded molecular duplexes.

H Zeng1, H Ickes, R A Flowers

  • 1Department of Chemistry, Natural Sciences Complex, State University of New York, Buffalo, NY 14260, USA.

The Journal of Organic Chemistry
|May 12, 2001
PubMed
Summary

Molecular duplexes with mismatched hydrogen bonds maintain structural integrity via remaining bonds. This demonstrates that molecular self-assembly is highly sequence-specific, establishing their role in molecular recognition.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A case of left ventricular apical hypoplasia].

Zhonghua xin xue guan bing za zhi·2026
Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026
Same author

[Perioperative management of a patient with Kennedy disease undergoing knee replacement: A case report].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2026
Same author

Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

Physical review letters·2025
Same author

Artificial rainfall patterns alter non-structural carbohydrate allocation to modulate growth and eco-stoichiometry in Cyphomandra betacea seedlings.

Plant biology (Stuttgart, Germany)·2025
Same author

[Application of plasmatrix in improving peri-implant soft tissue phenotype].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2025

Area of Science:

  • Supramolecular Chemistry
  • Chemical Biology
  • Materials Science

Background:

  • Hydrogen-bonded molecular duplexes are crucial for self-assembly and molecular recognition.
  • Understanding the impact of mismatched binding sites on duplex stability and assembly is key.

Purpose of the Study:

  • To design and synthesize hydrogen-bonded molecular duplexes with single mismatched binding sites.
  • To investigate the structural and thermodynamic consequences of these mismatches.
  • To establish the role of these duplexes in predictable molecular recognition.

Main Methods:

  • Synthesis of novel hydrogen-bonded molecular duplexes (1.3 and 1.4) with mismatched binding sites.
  • One- and two-dimensional Nuclear Magnetic Resonance (NMR) spectroscopy to assess structural integrity.

Related Experiment Videos

  • Isothermal Titration Calorimetry (ITC) to determine thermodynamic stability.
  • Competition experiments with complementary single strands.

    • Main Results:

    • Duplexes 1.3 and 1.4, despite single mismatches, maintained backbone register through residual hydrogen bonds.
    • Structural adjustments (twisting) occurred at mismatched sites to alleviate repulsive interactions.
    • Duplexes 1.3 and 1.4 were significantly less stable (>40 times) than the perfectly matched duplex 1.2.
    • Competition experiments showed displacement of mismatched duplexes by perfectly matched ones, indicating high sequence specificity.

    Conclusions:

    • The self-assembly of these hydrogen-bonded duplexes is highly sequence-specific, even with minor mismatches.
    • Mismatched binding sites lead to reduced duplex stability but do not disrupt overall structural integrity.
    • These duplexes serve as predictable and programmable molecular recognition units for directing intermolecular interactions.