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Jorge Lobo's disease: experimental inoculation in Swiss mice
D V Opromolla1, S Madeira, A F Belone
1Divisão de Pesquisa e Ensino, Instituto Lauro de Souza Lima, Bauru, SP, Brasil. pesquisa@ilsl.br
Revista Do Instituto De Medicina Tropical De Sao Paulo
|February 12, 2000
Summary
Swiss mice infected with Jorge Lobo's Disease fungi showed persistent infection but limited viability. This study suggests Swiss mice are not ideal for modeling this disease, though other strains may be susceptible.
Area of Science:
- Mycology
- Infectious Diseases
- Immunology
Background:
- Jorge Lobo's Disease is a chronic fungal infection.
- Understanding host-pathogen interactions is crucial for disease management.
Purpose of the Study:
- To evaluate Swiss mice as a model for Jorge Lobo's Disease.
- To investigate the host immune response and fungal persistence.
Main Methods:
- Intradermal inoculation of Swiss mice with fungal suspensions from patient biopsies.
- Quantification of fungal load and viability using Neubauer chamber and FD-EB staining.
- Histopathological analysis of inoculated foot pads over 18 months.
Main Results:
- Progressive cellular infiltrate, primarily macrophages containing fungi, observed.
- No macroscopic lesions developed, but Langhans' giant cells appeared after nine months.
- High fungal burden persisted, with low viability detected by FD-EB staining.
Conclusions:
- Swiss mice exhibit persistent fungal infection but limited fungal viability, suggesting macrophages destroy fungi but struggle with elimination.
- Swiss mice are not an ideal model for studying Jorge Lobo's Disease.
- Other mouse strains might be more susceptible and suitable for research.