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Recombinant mitochondrial DNA molecules suggest a template switching ability for group-II-intron reverse

C H Sellem1, O Begel, A Sainsard-Chanet

  • 1Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif sur Yvette, France. Sellem@cgm.cnrs-gif.fr

Current Genetics
|February 15, 2000
PubMed
Summary

Mitochondrial genome instability in Podospora anserina is linked to the mobile group-II intron COX1-i1. Its reverse transcriptase activity may cause DNA rearrangements during fungal aging and in mutants.

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Area of Science:

  • Molecular Biology
  • Mycology
  • Genetics

Background:

  • Degenerative processes in Podospora anserina correlate with mitochondrial genome instability.
  • The mobile group-II intron COX1-i1 (intron alpha) encodes a reverse transcriptase, contributing to this instability.

Purpose of the Study:

  • To characterize mitochondrial recombinant DNA molecules formed by intron alpha.
  • To investigate the role of reverse transcriptase activity in mitochondrial rearrangements.

Main Methods:

  • Polymerase Chain Reaction (PCR) experiments were employed.
  • Analysis of mitochondrial recombinant DNA molecules joining intron alpha 5' ends to tRNA 3' ends (including CCA motif).

Main Results:

  • Characterized recombinant DNA molecules with specific junction structures.

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  • Proposed that RNA template switching by the intron alpha-encoded reverse transcriptase initiated these junctions.

    • Conclusions:

    • The reverse transcriptase activity of COX1-i1 is a likely mechanism for mitochondrial rearrangements.
    • This mechanism may contribute to degenerative syndromes and observed phenotypes in long-lived mutants of P. anserina.