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Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard
H Summer1, T Pfannschmidt, G Link
1Arbeitsgruppe Pflanzliche Zellphysiologie und Molekularbiologie, Ruhr-Universität Bochum, Germany.
Abstract:
The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.