Related Experiment Video
Updated: Jun 28, 2026
07:00
A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects
Published on: August 14, 2013
Aqueous humor dynamics in monkeys with laser-induced glaucoma.
Summary
Laser-induced glaucoma in monkeys significantly increases intraocular pressure (IOP) due to reduced aqueous humor outflow facility. This ocular hypertension persisted long-term, despite an increase in uveoscleral outflow.
Area of Science:
- Ophthalmology
- Animal Models
- Glaucoma Research
Background:
- Glaucoma is characterized by elevated intraocular pressure (IOP).
- Understanding aqueous humor dynamics is crucial for glaucoma pathogenesis.
- Laser-induced glaucoma models provide insights into disease mechanisms.
Purpose of the Study:
- To investigate the long-term effects of laser-induced glaucoma on aqueous humor dynamics in cynomolgus monkeys.
- To determine the impact on intraocular pressure, aqueous flow, and outflow facility.
Main Methods:
- Laser photocoagulation was used to induce glaucoma in one eye of 18 cynomolgus monkeys.
- Measurements included intraocular pressure, aqueous flow, and outflow facility (tonography and fluorophotometry).
- Uveoscleral outflow was assessed using calculations and tracers.
Main Results:
- Laser-induced glaucoma eyes showed significantly increased IOP compared to control eyes.
- Aqueous flow was initially reduced but returned to baseline levels later.
- Both tonographic and fluorophotometric outflow facility were significantly reduced.
- Uveoscleral outflow increased, but not sufficiently to counteract IOP elevation.
Conclusions:
- Sustained reduction in outflow facility is the primary cause of increased IOP in this laser-induced glaucoma model.
- The compensatory increase in uveoscleral outflow is insufficient to prevent sustained ocular hypertension.
- This model demonstrates long-term aqueous humor dynamic alterations relevant to glaucoma.
Related Concept Videos
01:25Glaucoma: Overview
Glaucoma is an eye condition characterized by increased intraocular pressure that damages the retina and optic nerve, leading to irreversible blindness if left untreated. The human eye has various components, including the cornea, iris, pupil, lens, and optic nerve. Aqueous humor is secreted by the epithelium of the ciliary body in the posterior chamber and flows through the trabecular meshwork and canal of Schlemm, maintaining normal intraocular pressure. The trabecular meshwork and the canal...
01:27Open Angle Glaucoma: Treatment
In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...
Drugs such as carbonic anhydrase inhibitors, α2- and...
01:28Angle Closure Glaucoma: Treatment
Angle-closure glaucoma, or closed-angle glaucoma, is an eye condition where the iris bulges out and blocks the iridocorneal angle, resulting in a buildup of aqueous humor and increased intraocular pressure. Immediate medical attention is necessary due to the sudden onset of symptoms. The treatment for angle-closure glaucoma includes short-term and long-term approaches. Short-term treatment involves using eye drops like pilocarpine to lower intraocular pressure by increasing aqueous humor...