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Related Experiment Videos

Cross-reactivity among conifer pollens.

L A Schwietz1, D W Goetz, B A Whisman

  • 1Allergy-Immunology Department, Wilford Hall Medical Center, Lackland AFB, Texas, USA.

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
|February 16, 2000
PubMed
Summary
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Pollen from 12 Cupressaceae trees, including mountain cedar (MC), shows extensive cross-reactivity. The MC major allergen, gp40, is also cross-reactive with Japanese cedar, suggesting potential for component-based immunotherapy.

Area of Science:

  • Allergy and Immunology
  • Plant Science
  • Immunochemistry

Background:

  • Increasing reports of Cupressaceae pollinosis globally.
  • Cross-reactivity within the Cupressaceae family is suspected.
  • Juniperus ashei (mountain cedar) is a primary cause of South Texas respiratory allergies.

Purpose of the Study:

  • To investigate in vivo and in vitro cross-reactivity among 12 Cupressaceae species, one Taxodiaceae, one Pinaceae, and one angiosperm.
  • To compare pollen allergenicity of mountain cedar with other related and unrelated tree species.

Main Methods:

  • Skin prick testing with pollen extracts in patients with mountain cedar pollinosis.
  • Ouchterlony gel immunodiffusion using rabbit antisera to mountain cedar.
  • IgE immunoblotting and immunoblot inhibition assays with pooled human sera.

Related Experiment Videos

  • Monoclonal antibody immunoblotting targeting the gp40 major allergen of mountain cedar.

    • Main Results:

    • Positive skin reactions to all 12 Cupressaceae and Japanese cedar (Taxodiaceae) pollens.
    • Ouchterlony gels showed identity/partial identity between mountain cedar and Cupressaceae/Japanese cedar pollens.
    • IgE and monoclonal antibody binding confirmed cross-reactivity with major allergens in Cupressaceae and Japanese cedar.

    Conclusions:

    • Pollen proteins from 12 Cupressaceae species and Japanese cedar exhibit significant cross-reactivity.
    • The mountain cedar major allergen (gp40) is cross-reactive with allergens in other Cupressaceae and Japanese cedar.
    • Component-based immunotherapy could offer standardized treatment for Juniper and Cryptomeria japonica allergies.