Related Experiment Video

Updated: Aug 18, 2026

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment
07:45

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Published on: November 6, 2018

Insulinlike growth factor I plus insulinlike growth factor binding protein 3 attenuates the proinflammatory acute

M G Jeschke1, R E Barrow, D N Herndon

  • 1Shriners Hospital for Children and the Department of Surgery, University of Texas Medical Branch, Galveston 77550, USA.

Annals of Surgery
|February 16, 2000
PubMed

Insights

Insulinlike growth factor I (IGF-I) with IGFBP-3 reduced harmful inflammation in severely burned children. This treatment increased beneficial proteins and decreased inflammatory markers, potentially improving outcomes after severe burns.

Area of Science:

  • Biochemistry
  • Pediatric Critical Care
  • Metabolic Response to Injury

Background:

  • The hepatic acute phase response is crucial for homeostasis after trauma.
  • Prolonged acute phase response can lead to organ failure and mortality.
  • Understanding modulators of this response is vital for improving patient outcomes.

Purpose of the Study:

  • To investigate the impact of insulinlike growth factor I (IGF-I) combined with IGF binding protein 3 (IGFBP-3) on the hepatic acute phase response in severely burned children.
  • To assess the effects of IGF-I/BP-3 on serum protein levels and proinflammatory cytokines.

Main Methods:

  • A continuous infusion of IGF-I/BP-3 was administered to 22 severely burned children (mean TBSA burn 57%).
  • Eight control children received saline.
  • Serum samples were analyzed for constitutive hepatic proteins, acute phase proteins, and cytokines before and after treatment.

Main Results:

  • IGF-I/BP-3 administration significantly increased serum IGF-I and IGFBP-3 levels.
  • Constitutive hepatic proteins (prealbumin, RBP, transferrin) increased, while type I acute phase proteins (CRP, AAG, C3) decreased.
  • Proinflammatory cytokines (TNF-alpha, IL-1beta) decreased, with no change in IL-6.

Conclusions:

  • IGF-I/BP-3 treatment in severely burned children reduced IL-1beta and TNF-alpha, followed by a decrease in type I acute phase proteins.
  • This was associated with an increase in beneficial constitutive hepatic proteins.
  • Modulating the acute phase response with IGF-I/BP-3 may prevent organ failure and improve outcomes in thermal injury.
Abstract

More Related Videos

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice08:43

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Published on: January 31, 2020

A Swine Burn Model for Investigating the Healing Process in Multiple Depth Burn Wounds02:49

A Swine Burn Model for Investigating the Healing Process in Multiple Depth Burn Wounds

Published on: February 23, 2024

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Insulin: Dosing Regimen and Adverse Effects01:16

Insulin: Dosing Regimen and Adverse Effects

Insulin-replacement therapy usually includes both long-acting insulin (basal) and short-acting insulin (to cater to postprandial needs). In a diverse group of type 1 diabetes patients, the average daily insulin dose is typically 0.5-0.7 units/kg body weight. However, obese patients and pubertal adolescents may need more due to insulin resistance.
The basal dose constitutes about 40%-50% of the total daily dose, with the rest as premeal insulin. The mealtime insulin dose should mirror...
Acute Inflammation I: Inflammatory Response01:26

Acute Inflammation I: Inflammatory Response

Acute inflammation is a rapid, short-lived physiological response to tissue injury or infection, designed to eliminate harmful agents and initiate repair. This tightly regulated process typically lasts from minutes to several days and is triggered by factors such as microbial invasion, physical trauma, or chemical injury.Recognition and Mediator ReleaseThe inflammatory response begins when resident immune cells—such as mast cells, macrophages, and dendritic cells—detect damage-associated...
Acute Inflammation III: Local and Systemic Effects01:25

Acute Inflammation III: Local and Systemic Effects

Acute inflammation produces a coordinated set of local and systemic changes that limit injury, eliminate pathogens, and initiate repair. These responses arise within minutes of infection, trauma, or chemical insult and are driven by vascular alterations and leukocyte-derived mediators. When the stimulus resolves, the reaction typically abates within days.Local EffectsAt the site of injury, arteriolar vasodilation increases blood flow, resulting in redness and warmth. Simultaneously, increased...