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Selection of Pycnoporus cinnabarinus strains for laccase production
I Herpoël1, S Moukha, L Lesage-Meessen
1Laboratoire de Biotechnologie des Champignons Filamenteux, INRA, Centre d'Enseignement Supérieur en Biotechnologie, ESIL, 163 Avenue de Luminy, Case Postale 925, 13288, Marseille, France. herpoel@mailhost.esil.univ-mrs.fr
Abstract:
A comparison of Pycnoporus cinnabarinus strains for laccase production was carried out. A dikaryotic strain, I-937 strain, producing a high level of laccase (9500 U l(-1)) was selected. The study of the life cycle in vitro of this dikaryotic strain led to isolation of monokaryons. Forty-eight monokaryotic strains were isolated and screened for laccase production. One of these strains, ss3, produced a higher level of laccase than the parental strain I-937. The maximum production reached 29000 U l(-1) in medium supplemented with ferulic acid.