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Direct single-step surface plasmon resonance analysis of interactions between small peptides and immobilized
1Department of Organic Chemistry, University of Barcelona, Martí i Franquès 1-E-08028, Barcelona, Spain.
Journal of Immunological Methods
|February 17, 2000
Abstract:
Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibodies and peptides were observed. The kinetic data obtained provide further insight into the structure of the main antigenic site of foot-and-mouth disease virus (FMDV).