Related Experiment Videos
Cyclin C is a primary 1alpha,25-dihydroxyvitamin D(3) responding gene
P Polly1, C Danielsson, M Schräder
1Institut für Physiologische Chemie I, Heinrich-Heine-Universität, D-40001 Düsseldorf, Germany.
Journal of Cellular Biochemistry
|February 19, 2000
Summary
1alpha,25-dihydroxyvitamin D(3) (VD) upregulates cyclin C, a novel gene involved in cell cycle regulation. This finding offers new insights into VD
Area of Science:
- Endocrinology
- Molecular Biology
- Cell Biology
Background:
- 1alpha,25-dihydroxyvitamin D(3) (VD) is a nuclear hormone with known effects on cell cycle regulation.
- VD and its analogues inhibit cellular growth and induce apoptosis.
- The primary mediator genes responsible for these effects remain largely unknown.
Purpose of the Study:
- To identify novel VD target genes involved in cell cycle regulation.
- To investigate the role of cyclin C in VD-mediated cellular effects.
Main Methods:
- Differential display PCR (ddPCR) was used to screen for differentially expressed genes in MCF-7 cells treated with VD.
- Quantitative assessment of cyclin C mRNA and protein expression was performed.
- Cyclin C protein expression was validated in additional cell lines (MeWo and U937).
Main Results:
- Cyclin C was identified as a novel gene upregulated by VD and its analogues (EB1089, CB1093) in MCF-7 cells.
- Upregulation of cyclin C was observed at both mRNA and protein levels, with a more pronounced effect on protein expression.
- Increased cyclin C protein expression was confirmed in MeWo and U937 cell lines.
Conclusions:
- Cyclin C is a primary VD-responding gene.
- VD and its analogues upregulate cyclin C expression.
- Cyclin C's modulation of RNA polymerase II activity provides new insights into VD's mechanisms of cell cycle and transcriptional regulation.