[Study on certified reference material of germanium in Ganoderma lucidum]

Area of Science:

• Environmental Science
• Analytical Chemistry
• Pharmacognosy

Background:

• Accurate quantification of germanium (Ge) in Ganoderma lucidum is crucial for its applications in agriculture, forestry, medicine, and food hygiene.
• The lack of certified reference materials hinders precise Ge analysis and validation of analytical methods.

Purpose of the Study:

• To design and prepare a certified analytical reference material for germanium (Ge) in Ganoderma lucidum.
• To ensure accurate analysis, monitoring, and evaluation of Ge content in various trade sectors.
• To support technical training, method verification, and quality control for professional supervisors.

Main Methods:

• Development of a certified reference material for Ge in Ganoderma lucidum.
• Certification of the reference material using multiple analytical techniques: graphitic oven atomic absorption spectrometry, hydride spectrophotometry, polarography, chemical separation spectrophotometry, atomic fluorescence spectrometry, and X-ray fluorescence spectrometry.
• Statistical analysis of certified data using Grubb's law to confirm normal distribution and assess data normality.

Main Results:

• A certified analytical reference material for Ge in Ganoderma lucidum was successfully prepared.
• The material was validated using a suite of advanced analytical techniques.
• The certified crude data exhibited a normal distribution, with a mean value of 0.38 µg/g and a standard deviation of 0.08 µg/g.

Conclusions:

• The developed reference material provides a reliable standard for accurate Ge determination in Ganoderma lucidum.
• This certified material is essential for quality assurance in agricultural, medicinal, and food safety contexts.
• It facilitates inter-laboratory comparisons and enhances the reliability of Ge analysis in Ganoderma lucidum.