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[Identification and determination of Shenbaiye]
Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|February 23, 2000
Summary
This study developed a reliable method for identifying and quantifying Radix Sophorae flavescentis in Shenbaiye using Thin Layer Chromatography and titration. This ensures quality control for this traditional Chinese medicine.
Area of Science:
- Pharmacognosy
- Analytical Chemistry
- Traditional Chinese Medicine
Background:
- Shenbaiye is a traditional Chinese medicine formulation.
- Quality control is essential for ensuring the efficacy and safety of herbal medicines.
- Standardized methods are needed for the identification and quantification of active components in Shenbaiye.
Purpose of the Study:
- To establish a method for the identification and determination of Shenbaiye.
- To provide a basis for quality requirements of Shenbaiye.
- To quantify the active alkaloid content in Shenbaiye.
Main Methods:
- Thin Layer Chromatography (TLC) was used for the identification of three Chinese materia medica components: Radix Sophorae flavescentis (I), Cortex Phellodendri (II), and Rhizoma Atractylodis (III).
- Solid-phase extraction and neutral alumina column chromatography were employed for sample cleanup and separation.
- Titration with sulfuric acid and sodium hydroxide, using methyl red as an indicator, was used for quantification.
Main Results:
- TLC successfully identified the three components of Shenbaiye.
- A quantitative method was established for Radix Sophorae flavescentis (I), with a precision of 1.10% and recovery rates of 97.59%.
- The total alkaloid content was calculated as matrine.
Conclusions:
- The developed method is suitable for the identification and determination of Shenbaiye.
- The study provides a reliable analytical method for quality control of Shenbaiye.
- This research supports the standardization of Shenbaiye in traditional Chinese medicine practices.