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Updated: Aug 2, 2026
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Published on: May 21, 2015
Alterations in humoral immune responses associated with recurrent pregnancy loss
A C Eblen1, C Gercel-Taylor, L B Shields
1Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Kentucky, USA.
Objective:
To investigate the reactivity of maternal antibodies with endometrium-derived antigens and to correlate their association with recurrent pregnancy loss (RPL).
Design:
Prevalence study.
Setting:
Academic research center.
Patient(S):
Nulliparous women (n = 10), women with RPL (n = 15), pregnant women (n = 8), and multiparous women with a normal obstetric history (n = 20).
Intervention(S):
None.
Main Outcome Measure(S):
Reactive antibodies were analyzed by Western immunoblot techniques and quantitated by densitometry.
Result(S):
Antibodies from women with RPL and multiparous women recognized antigens ranging from 10-120 kd on normal endometrium and endometrial tumors. Antibodies from most women with RPL (10/15) and from multiparous women (15/20) recognized 65-kd and 80-kd proteins in normal endometrium. Antibodies from women with RPL recognized 21-kd and 28-kd antigens (12/15 and 13/15, respectively) in endometrial tumors at a significantly greater rate (than did antibodies from multiparous women (5/20 and 8/20, respectively). Women with RPL had significantly lower levels of asymmetric IgG compared with controls.
Conclusion(S):
Recurrent pregnancy loss may be linked with the failure to elicit asymmetric IgG and a unique immunologic recognition of endometrial antigens.
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