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Characterization of an inducible, epidermal-specific knockout system: differential expression of lacZ in different

T R Berton1, X J Wang, Z Zhou

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Genesis (New York, N.Y. : 2000)
|March 21, 2000
PubMed
Abstract

No abstract available in PubMed .

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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