Related Experiment Video
Updated: Sep 11, 2026
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
Ammonia concentration in bovine follicular fluid and its effect during in vitro maturation on subsequent embryo
D S Hammon1, S Wang, G R Holyoak
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 5600 Old Main Hill, Logan, UT 84332-5600, USA. hammon@cc.usu.edu
Abstract:
10 mm) to determine ammonia concentration. Ammonia concentration was greater (P<0.001) in the bFF from smaller follicles compared with bFF from larger follicles, and ammonia concentration decreased (P10 mm follicles, respectively. In Experiment 2, abattoir oocytes (n=2885) were matured in vitro in media containing ammonia at the concentrations of 0 microM (Control), 29 microM (A1), 88 microM (A2), 132 microM (A3), 176 microM (A4) and 356 microM (A5), followed by in vitro fertilization and culture. There was no difference (P0.05) in cleavage rates and morula or blastocyst development between treatments, indicating that in vitro exposure of bovine oocytes to ammonia does not adversely influence subsequent embryonic development.
