Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA microsatellite analysis using ion-pair reversed-phase high-performance liquid chromatography.

J M Devaney1, J E Girard, M A Marino

  • 1Transgenomic Inc., Gaithersburg, Maryland 20878, USA. jdevaney@transgenomic.com

Analytical Chemistry
|March 4, 2000
PubMed
Summary

Ion-pair reversed-phase high-performance liquid chromatography (IPRP HPLC) offers a faster, more efficient method for short tandem repeat (STR) DNA typing. This technique accurately analyzes STR alleles, reducing analysis time significantly compared to traditional methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portal vein thrombosis in patients with chronic liver diseases: From conventional to quantitative imaging.

European journal of radiology·2021
Same author

Non-AIDS defining cancers: a comprehensive update on diagnosis and management.

European review for medical and pharmacological sciences·2020
Same author

[Multiparametric and molecular imaging of breast tumors with MRI and PET/MRI].

Der Radiologe·2016
Same author

OS048 Mitochondrial content and function in placental cells and tissuesof preeclampsia and IUGR.

Pregnancy hypertension·2015
Same author

Genome-wide differentially methylated genes in prostate cancer tissues from African-American and Caucasian men.

Epigenetics·2015
Same author

Differences between conventional and nonconventional MRI techniques in Parkinson's disease.

Functional neurology·2013

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Short tandem repeat (STR) genotyping is crucial for human identification, parentage testing, gene mapping, and disease diagnostics.
  • Traditional slab gel electrophoresis for STR analysis is time-consuming and labor-intensive.
  • Alternative methods like capillary electrophoresis and ion-pair reversed-phase high-performance liquid chromatography (IPRP HPLC) are explored for DNA analysis.

Purpose of the Study:

  • To evaluate the efficacy of IPRP HPLC as a substitute for gel electrophoresis in STR analysis.
  • To optimize IPRP HPLC conditions for the accurate sizing and typing of STR alleles.
  • To assess the speed and efficiency of IPRP HPLC for STR locus analysis.

Main Methods:

  • Optimization of IPRP HPLC conditions, including column temperature, flow rate, and organic modifier gradient.

Related Experiment Videos

  • Separation and analysis of Polymerase Chain Reaction (PCR) products from the HUMTHO1 STR locus.
  • Sizing of HUMTHO1 alleles using internal markers under native (double-stranded) conditions.

    • Main Results:

    • Optimized IPRP HPLC conditions enabled efficient separation of PCR products.
    • Typing results for the HUMTHO1 locus showed 100% correlation with accepted DNA typing methods.
    • Analysis of the HUMTHO1 locus was completed in under 14 minutes, with potential for further allelic examination.

    Conclusions:

    • IPRP HPLC is a viable and efficient alternative to gel electrophoresis for STR analysis.
    • The method provides accurate sizing and typing of STR alleles with reduced analysis time.
    • IPRP HPLC offers advantages such as speed and elimination of hazardous detection systems for DNA typing applications.