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A simple HPLC method using a microbore column for the analysis of doxorubicin
1Department of Pharmaceutical and Administrative Sciences, School of Pharmacy and Allied Health Professions, Creighton University, Omaha, NE 68178, USA.
Journal of Pharmaceutical and Biomedical Analysis
|March 4, 2000
Summary
A new HPLC method accurately measures doxorubicin, a key bone cancer drug. This validated technique is crucial for studying doxorubicin release from drug delivery systems.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Doxorubicin is a potent anti-tumor agent widely used for bone cancer treatment.
- Accurate quantification of doxorubicin is essential for therapeutic efficacy and drug delivery system evaluation.
Purpose of the Study:
- To develop and validate a simple, sensitive, and rapid High-Performance Liquid Chromatography (HPLC) method for doxorubicin assay.
- To apply the validated HPLC method for assessing in vitro doxorubicin release from implantable drug delivery systems.
Main Methods:
- Utilized a C18 Luna microbore column with a fluorescent detector (505 nm Ex., 550 nm Em.).
- Employed an isocratic mobile phase (water-acetonitrile-acetic acid, 80:19:1, pH 3.0) at a flow rate of 0.1 ml/min.
- Validated the method for linearity, precision, accuracy, and detection limit using daunomycin as an internal standard.
Main Results:
- Achieved a detection limit of 0.02 ng (0.035 pmol/injection) with a 10-minute run-time.
- Demonstrated excellent linearity over the concentration range of 0.01-0.1 µg/ml.
- Reported high precision (R.S.D. < 4.0%) and accuracy (R.S.D. < 4.1%) for the doxorubicin assay.
Conclusions:
- The developed HPLC method is simple, sensitive, and suitable for accurate doxorubicin quantification.
- This validated method can be effectively utilized to study the in vitro release kinetics of doxorubicin from drug delivery devices.