Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diversity among high-level aminoglycoside-resistant enterococci.

J Papaparaskevas1, A Vatopoulos, P T Tassios

  • 1Microbiology Department, 'Laikon' General Hospital, Athens. Department of Hygiene and Epidemiology and Department of Microbiology, University of Athens Medical School, Athens, Greece. iosifp@otenet.gr

The Journal of Antimicrobial Chemotherapy
|March 7, 2000
PubMed
Summary

This study investigated antibiotic resistance in Enterococcus faecalis and Enterococcus faecium clinical isolates. High-level aminoglycoside resistance was common in E. faecalis, with specific genetic markers and dissemination patterns identified.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro activity of aztreonam/avibactam against a global collection of Klebsiella pneumoniae collected from defined culture sources in 2016 and 2017.

Journal of global antimicrobial resistance·2020
Same author

Comparison of Rappaport-Vassiliadis Enrichment Medium and Tetrathionate Brilliant Green Broth for Isolation of Salmonellae from Meat Products.

Journal of food protection·2019
Same author

Results from the Survey of Antibiotic Resistance (SOAR) 2014-16 in Greece.

The Journal of antimicrobial chemotherapy·2018
Same author

Bacterial next generation sequencing (NGS) made easy.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2018
Same author

Abnormal vaginal flora in symptomatic non-pregnant and pregnant women in a Greek hospital: a prospective study.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2016
Same author

Resistant Shigella strains in refugees, August-October 2015, Greece.

Epidemiology and infection·2016

Area of Science:

  • Clinical microbiology
  • Molecular epidemiology
  • Antimicrobial resistance

Background:

  • Enterococcus species are significant nosocomial pathogens.
  • Understanding their antimicrobial resistance patterns is crucial for effective treatment.
  • High-level aminoglycoside resistance (HLAR) is a concern in enterococcal infections.

Purpose of the Study:

  • To characterize the antimicrobial resistance profiles of Enterococcus faecalis and Enterococcus faecium isolates.
  • To investigate the genetic basis and molecular epidemiology of high-level aminoglycoside resistance.
  • To differentiate the spread and acquisition mechanisms of HLAR between E. faecalis and E. faecium.

Main Methods:

  • Phenotypic antibiotic susceptibility testing.
  • Detection of aminoglycoside resistance genes using Polymerase Chain Reaction (PCR).

Related Experiment Videos

  • Molecular typing of isolates using Pulse Field Gel Electrophoresis (PFGE).

    • Main Results:

    • No vancomycin resistance was detected; ampicillin resistance was not due to beta-lactamase production.
    • High-level gentamicin resistance (HLGR) was found in 22% of E. faecalis and 0% of E. faecium.
    • High-level streptomycin resistance (HLSR) was observed in 40% of E. faecalis and 33% of E. faecium.
    • Specific genes (aac(6")+aph(2") and ant(6)-I) were associated with HLAR.
    • PFGE revealed distinct E. faecalis clones with HLAR disseminated within the hospital.
    • E. faecium with HLAR appeared to be acquired nosocomially through diverse genetic types.

    Conclusions:

    • Molecular typing is essential for understanding the distinct epidemiological features of HLAR in E. faecalis and E. faecium.
    • E. faecalis HLAR strains showed clonal dissemination, while E. faecium HLAR was acquired through multiple independent events.
    • These findings highlight the importance of molecular surveillance for controlling enterococcal infections.