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Ultraviolet spectrometric determination of neutral monosaccharides by HPLC with ethanolamine
A Nakamura1, C Hatanaka, Y Nagamatsu
1Hannan R&D Center, Fuji Oil Co. Ltd., Osaka, Japan. 940080@so.fujioil.co.jp
Bioscience, Biotechnology, and Biochemistry
|March 8, 2000
Abstract:
The reaction between ethanolamine and sugars in a borate solution gave intense UV absorption at 310 nm. By adding ethanolamine in the mobile phase, simple and sensitive ion-exchange HPLC of sugar-borate complexes was achieved. This method can be used to measure as little as 100 pmole of seven monosaccharides from pectic polysaccharides.
