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Direct thin layer chromatography enantioresolution of some basic DL-amino acids using a pharmaceutical industry waste
R Bhushan1, J Martens, G T Thiongo
1Department of Chemistry, University of Roorkee, India. rbcy@rurkiu.ernet.in
Journal of Pharmaceutical and Biomedical Analysis
|March 9, 2000
Abstract:
Direct enantiomeric resolution of DL-arginine, DL-histidine, DL-lysine, DL-valine and DL-leucine into their enantiomers was achieved by thin layer chromatography (TLC) on silica gel plates impregnated with optically pure (1R, 3R, 5R)-2-azabicyclo[3,3,0]octan-3-carbo-xylic acid (0.011 M) as a chiral selector which is a waste of a pharmaceutical industry. Different combinations of acetonitrile-methanol-water were found to be successful in resolving the DL-amino acids. The spots were detected by ninhydrin (0.2% in acetone) and the detection limit was 0.66 microg.