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A validated quantitative colorimetric assay for gentamicin.

P Frutos1, S Torrado, M E Perez-Lorenzo

  • 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Complutense University Madrid, Spain. pafrutos@eucmax.sim.ucm.es

Journal of Pharmaceutical and Biomedical Analysis
|March 9, 2000
PubMed
Summary

A new colorimetric assay accurately quantifies gentamicin using the ninhydrin reaction. This simple, inexpensive method is sensitive and reproducible for gentamicin analysis.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Gentamicin is a crucial antibiotic requiring precise quantification.
  • Existing gentamicin quantification methods can be complex or costly.

Purpose of the Study:

  • To develop a simple, sensitive, and cost-effective colorimetric assay for gentamicin quantification.
  • To optimize the assay conditions for reliable gentamicin measurement.

Main Methods:

  • A colorimetric method utilizing the ninhydrin reaction with gentamicin.
  • Optimization of reaction parameters including pH, ninhydrin concentration, and reaction time.
  • Spectrophotometric measurement of color intensity at 400 nm.

Main Results:

  • The ninhydrin reaction with gentamicin produced a measurable purple color.

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  • A linear relationship was observed between absorbance and gentamicin concentration (30-120 µg/ml).
  • The optimized assay demonstrated reproducibility and sensitivity.

    • Conclusions:

    • A practical, inexpensive, and sensitive colorimetric assay for gentamicin quantification has been established.
    • The method is suitable for routine laboratory use due to its simplicity and reproducibility.