[Nitric oxide as a contrast modulator of basic elements of the cytoskeleton]

Early it was shown that nitric oxide induced in the cerebellum neuronal net both degenerative and compensatory-adaptive changes: 1) bouton encapsulation of spines, and 2) spiral wraps formed by glial cell processes around synapses and boutons. All these morphological changes were produced with cytoskeleton involvement. In the present work we have found that a NO-generative compound enhanced the contrast of cytoskeleton elements which depended on the concentration of this compound. The best contrast was observed at 1 mM concentration. The reason of the contrast enhance may be due presumably to protein transition from a soluble to a membrane-bound state. Using the contrast enhance effect we carried out a comparative analysis of cytoskeleton elements (CE) composition. Results of the analysis showed the specificity of CE in different cell structures: bouton, spine, glial cell. The obtained data support our proposal about the leading role of cytoskeleton in compensatory-adaptive morphological changes in extremal conditions.