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Immunomodulation by 8-week voluntary exercise in mice
H Sugiura1, H Nishida, R Inaba
1Department of Health and Physical Education, Gifu Pharmaceutical University, 5-6-1 Mitahora higashi, Gifu, Japan.
Acta Physiologica Scandinavica
|March 11, 2000
Summary
Voluntary exercise significantly boosts macrophage and lymphocyte immune functions in mice. This study recommends measuring nitric oxide (NO2-) production to assess macrophage activity in exercise research.
Area of Science:
- Immunology
- Exercise Physiology
- Cell Biology
Background:
- Immune cell function is crucial for overall health.
- The impact of voluntary exercise on immune cell activity requires further investigation.
Purpose of the Study:
- To evaluate the effects of voluntary exercise on macrophage and lymphocyte functions in mice.
- To determine if voluntary exercise enhances immune cell responsiveness.
Main Methods:
- Male A/He inbred mice were divided into exercise and control groups.
- Mice underwent voluntary wheel running for 8 weeks.
- Macrophage and lymphocyte functions were assessed via glucose consumption, enzyme activities, nitric oxide production, and cytokine release.
Main Results:
- Exercise group showed significantly higher macrophage glucose consumption and enzyme activities (acid phosphatase, beta-glucuronidase, lactate dehydrogenase).
- Macrophage nitric oxide (NO2-) production stimulated by lipopolysaccharide (LPS) was approximately doubled in the exercise group.
- Lymphocyte stimulation indices and interleukin-2 (IL-2) production were significantly elevated in mice that exercised voluntarily.
Conclusions:
- Voluntary exercise enhances both macrophage function and lymphocyte responsiveness in mice.
- Nitric oxide (NO2-) production is a recommended indicator for evaluating macrophage function in voluntary exercise studies.