Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plant protoplast isolation - a practical approach.

Prasad1, Naik

  • 1Department of Biotechnology, Gulbarga University, Gulbarga, India

Biochemical Education
|March 16, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reducing the Delay in the Diagnosis of Bipolar Disorder: A Qualitative Study.

Health expectations : an international journal of public participation in health care and health policy·2025
Same author

Standardization and bioavailabililty of ayurvedic drug lauha bhasma part-1 physical and chemical evaluation.

Ancient science of life·2012
Same author

Author's reply.

Histopathology·2000
Same author

Synthesis and analgesic activity of some novel 2-substituted 1,3,4-thiadiazolo

Arzneimittel-Forschung·2000
Same author

Fascinating Alkali Halide Structures of Different Dimensionalities Incorporated in Host Lattices.

Angewandte Chemie (International ed. in English)·2000
Same author

Intermittency transitions to strange nonchaotic attractors in a quasiperiodically driven duffing oscillator

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·2000
Same journal

Advances in experimental medicine and biology. Volumes 138-142 Published by Plenum Press, New York. Vol 138, $49.50; Vol 139, $59.50; Vol 140, $35; Vol 142, $49.50.

Biochemical education·2020
Same journal

The educational implications of biotechnology: A report on the seminar held at the National University of Singapore on June 5, 1981 by the IUBS Commission for Biological Education.

Biochemical education·2020
Same journal

A practical approach to the choice of a suitable detergent and optimal conditions for solubilizing a membrane protein.

Biochemical education·2000
Same journal

Oxidation of cholinesterase-inhibiting pesticides: A simple experiment to illustrate the role of bioactivation in the toxicity of chemicals.

Biochemical education·2000
Same journal

Carboxyfluorescein fluorescence experiments.

Biochemical education·2000
Same journal

A simple protocol to evaluate nitrogen utilisation in Saccharomyces cerevisiae.

Biochemical education·2000
See all related articles

This study presents an affordable method for isolating plant protoplasts using readily available enzymes. This technique is ideal for practical student training in biotechnology and plant science.

Area of Science:

  • Plant Biology
  • Biotechnology

Background:

  • Plant protoplast isolation is crucial for genetic engineering and cell biology research.
  • Current methods often rely on expensive or specialized enzyme preparations.

Purpose of the Study:

  • To develop an economical and accessible protocol for plant protoplast isolation.
  • To provide a practical method suitable for educational purposes in biotechnology.

Main Methods:

  • Utilized inexpensive, commercially available enzyme sources for cell wall digestion.
  • Optimized enzyme concentrations and incubation times for efficient protoplast release.

Main Results:

  • Successfully isolated viable plant protoplasts using the cost-effective enzyme method.

Related Experiment Videos

  • Demonstrated the suitability of the protocol for hands-on student laboratory exercises.

    • Conclusions:

    • The developed method offers a budget-friendly alternative for plant protoplast isolation.
    • This approach enhances practical learning experiences in plant biotechnology education.