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Characterization of an avidin-bonded column for direct injection in reversed-phase high-performance liquid
H Tanaka1, K Takahashi, M Ohira
1GL Sciences, Saitama, Japan. rd@gls.co.jp
Journal of Chromatography. A
|March 17, 2000
Abstract:
A denatured avidin-bonded column was suitable for use in the reversed-phase HPLC and complete recovery of serum protein over a wide range of pH. Retention property of the denatured avidin-bonded column was very nearly to a non-denatured avidin-bonded column already reported and, however, showed very high stability to organic solvent. A performance of the denatured avidin-bonded column was maintained even if 500 continuous injections of human serum (total 10 ml).