Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved kinetic parameter estimation in pH-profile data treatment.

I T Some1, P Bogaerts, R Hanus

  • 1Laboratoire de Chimie bioanalytique, de Toxicologie et de Chimie physique appliquée, Institut de Pharmacie, Université Libre de Bruxelles, Campus de la Plaine, CP 205/1 Boulevard du Triomphe, B-1050, Brussels, Belgium.

International Journal of Pharmaceutics
|March 21, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soft tissue vascular tumor-like lesions in adults: imaging and pathological analysis pitfalls per ISSVA classification.

Insights into imaging·2024
Same author

Development of cortical folds in the human brain: An attempt to review biological hypotheses, early neuroimaging investigations and functional correlates.

Developmental cognitive neuroscience·2023
Same author

Depletion of CLL cells by venetoclax treatment reverses oxidative stress and impaired glycolysis in CD4 T cells.

Blood advances·2022
Same author

Characterization of suicidal depression: a one-year prospective study.

European psychiatry : the journal of the Association of European Psychiatrists·2022
Same author

Predictors of mortality, ICU hospitalization, and extrapulmonary complications in COVID-19 patients.

Infectious diseases now·2021
Same author

Nonadiabatic effects in the double ionization of atoms driven by a circularly polarized laser pulse.

Physical review. E·2020
Same journal

Preventing tablet defects through vacuum-assisted deaeration of a powder bed.

International journal of pharmaceutics·2026
Same journal

Approaches for enhancing bioavailability of macromolecular drugs.

International journal of pharmaceutics·2026
Same journal

Characteristics of asymmetric microcrystalline solidification pellets and a better prediction for bioequiavailability based on solubility-permeability theory.

International journal of pharmaceutics·2026
Same journal

A CFPD-FSI analysis of the impact of nasal hairs on airflow patterns, nasal resistance, and particle filtration in a realistic human nasal airway.

International journal of pharmaceutics·2026
Same journal

Transient hydrate formation during disintegration of film-coated pharmaceutical tablets.

International journal of pharmaceutics·2026
Same journal

Erratum to "In vitro - In vivo correlation modeling of an oxcarbezapine extended-release formulation using numerical deconvolution" [Int. J. Pharm. 699 (2026) 126952].

International journal of pharmaceutics·2026
See all related articles

This study introduces a novel one-step method to improve statistical analysis in pH-profile parameter estimation for drug stability. The new approach offers a more rigorous statistical viewpoint for kinetic and dissociation constant determination.

Area of Science:

  • Pharmaceutical Sciences
  • Physical Chemistry
  • Chemical Kinetics

Background:

  • Statistical challenges in parameter estimation are well-documented for temperature stability studies.
  • Limited attention has been given to statistical issues in pH-profile parameter estimation.
  • Conventional two-step methods in data treatment present similar statistical problems for both temperature and pH stability.

Purpose of the Study:

  • To introduce an improved statistical method for pH-profile parameter estimation.
  • To address the statistical shortcomings of conventional two-step methods in drug stability analysis.
  • To develop a more rigorous approach for determining kinetic and dissociation constants.

Main Methods:

  • A one-step non-linear method was developed, incorporating errors in drug content determination.

Related Experiment Videos

  • A mathematical relationship linking drug content (C), pH, and time (t) was established and tested.
  • Kinetic data from acetylsalicylic acid (ASA) hydrolysis were utilized for method validation.

    • Main Results:

    • The proposed method enables simultaneous estimation of specific kinetic constants and the dissociation constant (pK(a)) in a single experimental run.
    • It also determines the most likely experimental initial drug contents (C(0j)) for each experiment.
    • The one-step approach provides a statistically more rigorous estimation of kinetic parameters compared to traditional two-step methods.

    Conclusions:

    • The developed one-step method significantly enhances the statistical rigor of pH-profile parameter estimation.
    • This approach offers a more comprehensive analysis by considering all relevant experimental data.
    • The method is applicable to drug stability studies, providing accurate determination of kinetic and dissociation constants.