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Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
Published on: November 12, 2017
Electrochemical studies of the interaction of adriamycin to DNA
Abstract:
An electrochemical investigation of the interaction of adriamycin (DXH) with DNA on a Hg electrode is reported. In weakly acidic media of pH 4.0-7.0, the addition of DNA to DXH solution results in the decrease of redox peak currents. In the presence of DNA, no new peak appears and the standard rate constant k(s) is not significantly changed. The binding of DXH to DNA by electrostatic attraction and intercalation forms a kind of supramolecular complex DXH-DNA, which is electrochemically non-active. The equilibrium constant for the complex is calculated. The decrease in peak current is proportional to DNA concentration and can be used to determine DNA concentration.

